Mastercycler® X50

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Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50

The new Mastercycler X50 is the elegant synthesis of speed, flexibility and PCR optimization for research PCR applications and standardization for routine PCR applications such as food testing etc. A new highly intuitive touch screen concept puts all of those benefits always at your fingertips. More information

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Product Information

Speed and enhanced PCR optimization functions like the 2D-Gradient make the Mastercycler X50 the ideal PCR cycler for advanced research in molecular biology. The excellent block temperature regulation gives rise to the next stage of PCR reproducibility, whereas the adaptable user management and profound documentation capabilities give peace of mind to laboratories working to set standards.
A highly intuitive touch display, low noise levels, low power consumption, and the versatile flexlid® concept complete the product to be a powerful PCR cycler. Up to 10 PCR cyclers can be combined – ideal for high throughput applications or labs with a high number of users running different assays. Should you feel you need more flexibility or throughput, up to 50 PCR cyclers can be combined in a computer-controlled network.

PCR Optimization of β-actin Gene with 2D-Gradient technique.


2D-Gradient allows you to optimize both the denaturation temperature (bottom to top) and the annealing temperature (left to right) during the same run. Higher denaturation temperatures can have the advantage of increased specificity while lower denaturation temperatures reduce stress on biomolecules and can lead to increased yield. Assays that struggle to work reliably at a 95° denaturation temperature could benefit significantly from the optimization of the denaturation temperature.

Mastercycler® X50

PCR should be faster. That gives you quicker results, throughout the day, and a faster transition to optimal temperatures. The Mastercycler X50 heats with 10 °C/s and cools with 5 °C/s. Make the speed of the Mastercycler X50 your research advantage.

Mastercycler® X50

There ia a large variety of cyclers available with different ramp rates published.
If you compare the corresponding run times by performing the same protocol using different cyclers, you see that ramp rates do not tell the full story about whether a cycler is fast or not. For more details, see Application Note 274.

Mastercycler® X50

Features

  • Innovative 2D-Gradient for advanced PCR optimization
  • Heating rate: up to 10 °C/s
  • Wide selection of blocks from a fast silver block to 384
  • Intuitive touch display
  • Connect up to 10 units to a network
  • flexlid® concept: automatic height adjustment of the lid allows you to use all types of consumables
  • Small footprint
  • 2 year warranty

Applications

  • Fast PCR
  • PCR optimization
  • Standard PCR
  • Incubation
  • Cycle sequencing
 MC X50sMC X50aMC X50pMC X50hMC X50iMC X50lMC X50rMC X50t
ThermoblockSilverAluminumAluminumAluminumSilverAluminumAluminumAluminum
High pressure lid
96-well plate
384-well plate
0.1/0.2 mL tubes
Temperature control range of the block4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C
Temperature control modeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, Safe
Heating technologySix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elements
2D-Gradient blockover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 24 columns/over 16 rowsover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 24 columns/over 16 rows
Gradient range1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C
Gradient temperature range30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C
Lid temperature range37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C
Lid descentflexlidflexlidflexlidflexlidflexlidflexlidflexlidflexlid
Block homogeneity 20–72 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C
Block homogeneity 95 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C
Block temperature accuracy±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C
Heating rate10 °C/s5 °C/s5 °C/s5 °C/s10 °C/s5 °C/s5 °C/s5 °C/s
Cooling rate5 °C/s2.3 °C/s2.3 °C/s2.3 °C/s5 °C/s2.3 °C/s2.3 °C/s2.3 °C/s
InterfacesEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USB
Total weight11.5 kg11.5 kg11.5 kg11.5 kg10.7 kg10.7 kg10.7 kg10.7 kg
Power supply110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz
Max. power consumption850 W850 W850 W850 W850 W850 W850 W850 W
Dimensions (W × D × H)27.5 × 43 × 33 cm27.5 × 43 × 33 cm27.5 × 43 × 33 cm27.5 × 43 × 33 cm27.5 × 43 × 33 cm27.5 × 43 × 33 cm27.5 × 43 × 33 cm27.5 × 43 × 33 cm

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