Tired of condensation in your tubes? We combined the Eppendorf ThermoMixer® with the PCR cycler approach of a heated lid and generated the Eppendorf ThermoTop. The outstanding Eppendorf condens.protect technology reliably prevents condensation within the tube lid – sample concentrations are consistent for the incubation time. In addition, the temperature homogeneity is further improved. Get optimal sample reaction conditions to produce optimal results.
The ThermoTop impresses with a simple, wireless handling as well as the automatic recognition and operation. It is detected by the device as soon as it is positioned, via inductive signal transmission, and thermoregulation starts automatically. The temperature optimally adapts to the test temperature to ensure that it does not negatively affect the sample. Condensation droplets have no opportunity to form.
Without the use of a heated lid, solvent molecules will evaporate to the gaseous phase during the heating process of a liquid sample and subsequently condensate against the cooler surfaces of lid and container wall. Hence, the volume of the sample is reduced while its concentration increases (fig. A).
Sensitive reactions may be influenced by this change in reaction conditions. In the case of very small sample volumes, the condensation process can leave such a small residual sample volume inside the reaction tube that the sample is no longer mixable. Under such conditions the biochemical reaction will be neither optimal nor reproducible.
When using the Eppendorf ThermoTop, the surfaces of the tube lids and upper tube walls are warmed in such a way as to prevent the formation of condensation effectively.
Thus, sample concentration remains stable, reaction conditions are strictly adhered to, and reproducibility of experiments is improved (fig. B).
The ThermoTop is compatible with the ThermoMixer C, ThermoStat C, ThermoMixer F0.5/ F1.5/ F2.0 and ThermoMixer FP. Combined with the ThermoMixer C and the ThermoStat C, the ThermoTop can be used with thermoblocks for plates and lab vessels with a volume up to 2 mL. Compatible thermoblocks feature the condens.protect symbol.