BioBLU^® c Single-Use Vessels

Made from monolayer virgin plastics, the Eppendorf BioBLU Single Use Vessels address the widely discussed problems associated with leachables and extractables (L&E), helping to make laboratories more efficient and safe. The rigid wall stirred–tank design mimics traditional vessels and makes scale-up easy. More information

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BioBLU^® 0.3c Single-Use Vessel, cell culture, open pipe, 1 pitched-blade impeller, no pH, sterile, 4 pieces Catalog No. 78903508

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BioBLU^® 0.3c Single-Use Vessel, cell culture, open pipe, 1 pitched-blade impeller, optical pH, sterile, 4 pieces Catalog No. 78903507

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BioBLU^® 1c Single-Use Vessel, cell culture, open pipe, 2 pitched-blade impellers, no pH, sterile, 4 pieces Catalog No. 1386110100

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BioBLU^® 1c Single-Use Vessel, cell culture, open pipe, 2 pitched-blade impellers, optical pH, sterile, 4 pieces Catalog No. 1386110500

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BioBLU^® 1c Single-Use Vessel, cell culture, open pipe, 1 pitched-blade impeller, no pH, sterile, 4 pieces Catalog No. 1386110000

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BioBLU^® 1c Single-Use Vessel, cell culture, open pipe, 1 pitched-blade impeller, optical pH, sterile, 4 pieces Catalog No. 1386110400

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BioBLU^® 3c Single-Use Vessel, cell culture, microsparger, 1 pitched-blade impeller, optical pH, sterile, 1 piece Catalog No. 1386000100

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BioBLU^® 3c Single-Use Vessel, cell culture, macrosparger, 1 pitched-blade impeller, optical pH, sterile, 1 piece Catalog No. 1386000300

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BioBLU^® 3c Single-Use Vessel, cell culture, microsparger, 2 pitched-blade impellers, optical pH, sterile, 1 piece Catalog No. 1386120000

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BioBLU^® 3c Single-Use Vessel, cell culture, macrosparger, 2 pitched-blade impellers, optical pH, sterile, 1 piece Catalog No. 1386121000

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BioBLU^® 10c Single-Use Vessel, cell culture, macrosparger, 1 pitched-blade impeller, optical pH, sterile, 1 piece Catalog No. 1386141000

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BioBLU^® 10c Single-Use Vessel, cell culture, microsparger, 1 pitched-blade impeller, optical pH, sterile, 1 piece Catalog No. 1386140000

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BioBLU^® 50c Single-Use Vessel, cell culture, microsparger, 1 pitched-blade impeller, optical pH, sterile, 1 piece Catalog No. M1363-0131

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BioBLU^® 50c Single-Use Vessel, cell culture, macrosparger, 1 pitched-blade impeller, optical pH, sterile, 1 piece Catalog No. M1363-0129

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BioBLU^® 5c Single-Use Vessel, cell culture, microsparger, 1 pitched-blade impeller, optical pH, sterile, 1 piece Catalog No. M1363-0125

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BioBLU^® 5c Single-Use Vessel, cell culture, macrosparger, 1 pitched-blade impeller, optical pH, sterile, 1 piece Catalog No. M1363-0121

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BioBLU^® 5p Single-Use Vessel, cell culture, microsparger, packed-bed impeller, optical pH, sterile, 1 piece Catalog No. M1363-0119

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BioBLU^® 5p Single-Use Vessel, cell culture, macrosparger, packed-bed impeller, optical pH, sterile, 1 piece Catalog No. M1363-0133

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BioBLU^® 14c Single-Use Vessel, cell culture, microsparger, 1 pitched-blade impeller, optical pH, sterile, 1 piece Catalog No. M1363-0126

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BioBLU^® 14c Single-Use Vessel, cell culture, macrosparger, 1 pitched-blade impeller, optical pH, sterile, 1 piece Catalog No. M1363-0122

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Product Information

Eppendorf BioBLU Single-Use Vessels combine all the advantages of single-use technology with the trusted performance and scalability of a stirred-tank design. Single-use vessels eliminate autoclaving, improve turn-around time, simplify validation, and reduce overall costs. The BioBLU portfolio has grown to accommodate users from early research and development through manufacturing. BioBLU c and p vessels cover a wide variety of processes, including shear-sensitive suspension cell cultures and adherent cell line development.

Features

Single-use, stirred-tank, rigid-walled vessels available in sizes ranging from 100 mL to 40 L working volume
All BioBLU c vessel sizes available with pitched-blade impellers for cell culture applications
BioBLU 5p vessels with Eppendorf-exclusive packed-bed impeller, pre-loaded with Fibra-Cel^® Disks
Unique non-invasive pH and DO sensor technology drastically reduces contamination risks (industry standard autoclavable pH sensors available for pH measurement)
Sealed magnetic drive with fully enclosed bearings maintain vessel sterility
Vessels are assembled with sparger, overlay, gas filters for inlet and exhaust as well as penetrations for pH, DO, temperature, liquid additions, sampling, and harvest
For use with Eppendorf benchtop bioreactor systems
Adaptor kits for legacy bioreactor controllers available
Technical and material documentation available to support process validation activities

Applications

Insect, mammalian, and human suspension cell lines
Stem cells
Adherent cells

	BioBLU^® 0.3c	BioBLU^® 1c	BioBLU^® 3c	BioBLU^® 10c	BioBLU^® 50c	BioBLU^® 5p
Standard set-up	DASbox	Bioblock/Benchtop	Benchtop	Benchtop	Benchtop	Benchtop
Bioreactors
Sterilization	Pre-sterilized (15 kGy β-irradiated)	Pre-sterilized (15 kGy β-irradiated)	Pre-sterilized (15 kGy β-irradiated)	Pre-sterilized (15 kGy β-irradiated)	Pre-sterilized (15 kGy β-irradiated)	Pre-sterilized (15 kGy β-irradiated)
Working volume (total)	100 – 250 mL (380 mL)	320 mL – 1.25 L (1.8 L)¹	1.25 – 3.75 L (5 L)	3.3 – 10 L (13.3 L)	18 – 40 L (50 L)	3.75 L (5 L)
Material vessel³	PS, PC (USP Class VI)	PS, PC (USP Class VI)	PC (USP Class VI)	PC (USP Class VI)	PS, PC (USP Class VI)	PS, PC (USP Class VI)
Material tubing	Silicone, C-Flex²	Silicone, C-Flex²	Silicone, C-Flex	Silicone, C-Flex	Silicone, C-Flex	Silicone, C-Flex
Head plate ports	2x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 1x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust	3x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 2x liquid addition overlay, 2x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust	4x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust	4x Pg 13.5 (1x 19 mm to Pg 13.5), 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust	1x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust	1x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 3x liquid addition overlay, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x gas overlay, 1x exhaust
Agitation
Drive	Magnetic overhead drives	Magnetic overhead drives	Magnetic overhead drives	Magnetic overhead drives	Magnetic overhead drives	Magnetic overhead drives
Impellers	Pitched-blade	Pitched-blade	Pitched-blade	Pitched-blade	Pitched-blade	Packed-bed
Recommended agitation speed	20 – 500 rpm	30 – 600 rpm	25 – 200 rpm	25 – 200 rpm	25 – 150 rpm	25 – 200 rpm
Gassing
Gas supply	Sparger and/or overlay	Sparger and/or overlay	Sparger and/or overlay	Sparger and/or overlay	Sparger and/or overlay	Sparger and/or overlay
Monitoring and Control
Sensor length	120 mm	220 mm	225 mm	355 mm	526 mm	120 mm
Temperature sensor	Pt100 RTD (not included)	Pt100 RTD (not included)	Pt100 RTD (not included)	Pt100 RTD (not included)	Pt100 RTD (not included)	Pt100 RTD (not included)
Max. operating temperature	40 °C	40 °C	40 °C	40 °C	40 °C	40 °C
pH sensor	Optical pH or standard glass sensor (120 mm)	Optical pH or standard glass sensor (220 mm)	Optical pH or standard glass sensor (220 mm)	Optical pH or standard glass sensor (325 mm)	Optical pH or standard glass sensor (625 mm)	Optical pH or standard glass sensor (120 mm)
DO Sensor	Polarographic DO - 4.7/118 mm	Polarographic DO - 4.7/229 mm	Polarographic or optical DO - 12/225 mm	Polarographic or optical DO - 12/355 mm	Polarographic DO - 12/526 mm	Polarographic DO - 12/120 mm
Exhaust condensation	Liquid-free cooling (Peltier)	Liquid-free cooling (Peltier)/water-cooled	Electric heater band or Peltier	Electric heater band	Electric heater band	Electric heater band or Peltier

¹425 mL minimal working volume when used with vessel stand and heating blanket
²C-Flex is for optical pH version only.
³PC = Polycarbonate, PS = Polystyrene

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